TitleCalpain 2 is required for glioblastoma cell invasion: regulation of matrix metalloproteinase 2.
Publication TypeJournal Article
Year of Publication2010
AuthorsJang HSang, Lal S, Greenwood JA
JournalNeurochem Res
Volume35
Issue11
Pagination1796-804
Date Published2010 Nov
ISSN1573-6903
KeywordsCalpain, Cell Movement, Gene Knockdown Techniques, Glioblastoma, Humans, Matrix Metalloproteinase 2, Neoplasm Invasiveness, RNA, Small Interfering
Abstract

Invasion of glioblastoma cells significantly reduces the effectiveness of current treatments, highlighting the importance of understanding dispersal mechanisms and characteristics of the invasive population. Induction of calcium fluxes into glioblastoma cells by autocrine glutamate is critical for invasion. However, the target(s) by which calcium acts to stimulate the dispersal of glioblastoma cells is not clear. In this study, we tested the hypothesis that the calcium-activated protease calpain 2 is required for glioblastoma cell invasion. Knockdown of calpain 2 expression using shRNA or chemical inhibition of calpain activity reduced glioblastoma cell invasion by 90%. Interestingly, decreased expression of calpain 2 did not influence morphology or migration, suggesting regulation of invasion specific mechanisms. Consistent with this idea, 39% less extracellular MMP2 was measured from knockdown cells identifying one mechanism by which calpain 2 mediates glioblastoma cell invasion. This is the first report demonstrating that calpain 2 is required for glioblastoma cell invasion.

DOI10.1007/s11064-010-0246-8
Alternate JournalNeurochem. Res.
PubMed ID20730561
PubMed Central IDPMC3006191
Grant ListP30 ES000210 / ES / NIEHS NIH HHS / United States
R01 GM063711 / GM / NIGMS NIH HHS / United States
GM 63711 / GM / NIGMS NIH HHS / United States
P30 ES00210 / ES / NIEHS NIH HHS / United States