The Seahorse Extracellular flux analyzer is located in room 2111A. This instrument provides an intermediate-throughput platform for rapid and sensitive identification of environmental chemicals altering cellular bioenergetics by measuring the two major energy producing pathways of the cell, mitochondrial respiration and glycolysis, in real-time.  The Analyzer provides a 96-well format to simultaneously perform time-resolved measurements of the oxygen consumption rate and extracellular acidification rate in a single population of cells over a period of minutes, hours, or even days.  The label-free assay system allows reuse of the cells following measurement.  Instrument software guides the user through the experimental design and provides a summary report on completion.

 

Seahorse Manual
ECAR Glycolysis Stress Test Kit Manual
OCR Cell Mito Stress Test Kit Manual