The Tissue Culture Core consists of a 4-room suite capable of complying with BSL2 safety requirements. Three of the rooms are equipped with laminar-flow hoods, incubators, water baths, tabletop centrifuges, phase and fluorescence microscopes, and a refrigerator and are appropriate for all types of tissue culture work. Two of the rooms also include chemical fume hoods. The Seahorse Extracellular Flux Analyzer and the xCELLigence cell monitoring systems are housed in these rooms. The fourth room houses the zebrafish disssection equipment and the High Content Imager as well as a refrigerator/freezer. The facility contains a Beckman Coulter Allegra X-15R refrigerated centrifuge with an SX4750 hanging bucket rotor that will spin plates, conicals, and some bottles. There is an en-suite autoclave available. The core maintains cryogenic dewars for long-term storage of cells in liquid nitrogen.
Permission to schedule the Google calendars is administered through the facility. Please contact the facility director or manager to enable this ability.
This room houses the Seahorse Extracellular Flux Analyzer and has a TC hood, a horizontal laminar flow hood, centrifuge, microscope, and refrigerator. Incubators are available for traditional 5% CO2 tissue culture and Seahorse culture equilibration (no CO2).
This room houses the Zeiss AxioCam Cell Photography system and has a TC hood, fume hood, centrifuge, microscope, refrigerator, and traditional 5% CO2 incubators.
This room houses both the High Content Imager and the mammalian protein production capability and has a TC hood, microscope, refrigerator/freezer, a traditional 5% CO2 incubator, and an incubator with the shaker for the mammalian cultures.
This room houses the zebrafish dissection equipment and a temporary incubator for transient incubation while dissecting, as well as another laminar dissection station and the fume hood.
This room will house the primary tissue culture capability and will have a TC hood, microscope, refrigerator/freezer, and traditional 5% CO2 incubators.